中药消臌方利水消臌作用的机制研究

To Study the mechanism of Traditional Chinese Medicine (TCM) on the ascites drainage

目的 观察中药消臌方 (简称方剂 )的利水消臌作用并探讨作用机制。方法 中药提取 ;尿液Na+ 测定 ;Griess法血清NO浓度测定 ;膈腹膜扫描电镜制样及计算机图像处理系统定量分析腹膜淋巴孔。结果 中药消臌方剂能提高尿液Na+ 的排泄 (P <0 .0 1) ;能增加膈腹膜淋巴孔面积、密度和周长 (P <0 .0 1) ;中药消臌方剂诱导的小鼠血清一氧化氮 (NO)升高能被一氧化氮合酶 (NOS)抑制剂 (L 硝基精氨酸 )抑制 (P <0 .0 1)。

OBJECTIVE To study the mechanism of traditional chinese medicine (TCM) on the ascites drainage. METHOD The extract of traditiona chinese medicine, The measurement of the concentration of sodium and NO in the serum. Scanning electron microscope and computer image processing were used to analysis the peritoneal lymphatic stomata.RESULTS Compared to N.S group and model group,TCM could significantly enhance the drainage of sodium ( P <0.01), and increase the perimeter and the area of the peritoneal lymphatic stomata,promote its distribution density ( P <0.01) and increase the concentration of NO. The effect of TCM on the peritoneal lymphatic stomata and the concentration of sodium was altered by adding NO supplier or NOS suppressor(L LMNNM) to the peritoneal cavity.CONCLUSION The observations indicated that TCM might regulate the peritoneal lymphatic stomata to drain materials in the peritoneal cavity by activating nos to induce the concentration of internal NO.