高效液相色谱法测定口腔愈溃膜中盐酸小檗碱

Determination of berberine hydrochloride in Kouqiang Yukuimo by HPLC

目的 建立高效液相色谱法测定中药口腔愈溃膜中盐酸小檗碱的含量。方法 用DiamonsilODS(4.6mm× 2 5 0mm ,5 μm)色谱柱 ,0 .0 3mol/LH3 PO4 乙腈 (72 :2 8,三乙胺调 pH3.0 )为流动相 ,流速 1.0mL/min ;检测波长 :347nm。 结果 盐酸小檗碱在 0 .2～ 1.5 μg/mL范围线性关系良好 ,平均回收率为 95 .73% ,符合分析要求。重现性试验的RSD为 0 .5 7%。 结论 对样品的提取条件和测定条件进行了优选 。

OBJECTIVE An HPLC method was developed for determination of Berberine in Kouqiang Yukuimo. METHOD The column was ODS Diamosil C18 (4.6mm×250mm); mobile phase was 0.03 mol/L phosphoric acid acetonitrile (72 ∶ 28, Adjust pH to 3.0 by Triethlamine). Detection wavelength was set at 347nm.RESULTS Linearity range was 0.2～1.5ug/mL(r=0.9993); The average recovery was 95.73% (RSD 0.97%). CONCLUSION The method is accurate and can be used for quality control of the preparation.