肝硬化组织微卫星改变的临床研究

The clinical study of microsatellite alteration of liver cirrhosis

目的 研究微卫星改变 (microsatellitealteration ,MA)在肝硬化组织的发生情况及其与病毒性肝炎的关系。方法 运用聚合酶链式反应 -简单重复序列多态性方法 (PCR SSLP)对 30例肝硬化组织石蜡标本的微卫星不稳定性 (microsatelliteinstability ,MSI)和等位基因杂合性丢失 (lossofheterozygosity ,LOH)进行研究。 结果 30例肝硬化组织中有 17例出现了MA(5 6 .7% ) ,其中至少有一个位点发生的MSI有 4例 (13.3% ) ,而有 15例至少有一个位点出现了LOH(5 0 % ) ,MA的发生与HBV感染及是否合并HCV感染无显著相关。

Objective To investigate the frequency of microsatellite alteration (MA) in cirrhotic livers and explore its association with viral hepatitis. Methods The loss of heterozygosity (LOH) and microsatellite instability (MSI) were analysed in 30 paraffin embedded cirrhotic tissues by polymerase chain reaction simple sequence length polymorphism (PCR SSLP). Results Out of 30 cirrhotic tissues, MA was detected in 17 (56.7%) , MSI was observed in 4 and LOH was observed in 15 at one or more loci. No significant correlation was found between MA and HBV and HCV infection. Conclusion MA occurs in cirrhosis as the early lesion of hepatocellular carcinoma.