乳腺癌中端粒酶活性与细胞凋亡的研究

Telomerase activity and apoptosis of breast cancer

目的 探讨端粒酶活性及细胞凋亡与乳腺癌生物学行为的关系。方法 分别采用端粒序列重复扩增法(TRAP法 )和末端脱氧核苷酸转移酶介导缺口末端标记 (TUNEL)法对 6 8例乳腺癌标本进行端粒酶活性和细胞凋亡的检测 ,并结合临床资料进行分析。结果 6 8例乳腺癌标本端粒酶活性阳性率为 88.2 % (6 0 / 6 8) ,细胞凋亡指数为 (5 6 .7± 15 .3) % ;端粒酶活性强度及细胞凋亡指数与患者的年龄无关 (P >0 .0 5 ) ,与肿瘤的大小、分级、分期、腋淋巴结转移数、激素受体状况有关 (P <0 .0 1) ,即端粒酶活性越高和 (或 )细胞凋亡越少 ,则肿瘤分化程度越低 ,提示预后越差。端粒酶活性强度与细胞凋亡指数呈明显的负相关 (r=- 0 .6 7,P <0 .0 1)。结论 乳腺癌端粒酶活性及细胞凋亡与肿瘤大小、分级、分期、淋巴结转移数和激素受体状况有关 ,端粒酶活性及细胞凋亡的检测有助于乳腺癌的临床分析 。

Objective To explore the relation between clinicobiological acting, telomerase activity and apoptosis of breast cancer. Methods The intensity of telomerase activity and apoptosis of breast cancer were detected by telomeric repeat amplification protocol(TRAP) and terminal deoxynucleotidyl transferase mediated dUTP biotin nick ending labeling (TUNEL) assay. Results The positive rate of telomerase activity of breast cancer was 88.2%, and the apoptosis index of breast cancer was (56.7±15.3)%. The intensity of telomerase activity and apoptosis of breast cancer were not related to patients' age ( P >0.05), but to the size, stage, grade of tumor,lymph node metastasis of axillary fossa, state of hormone receptor of breast cancer( P <0.01). The greater the intensity of telomerase activity or less apoptosis, the higher the grade or stage of tumor, suggesting the poor prognosis of breast cancer. The intensity of telomerase activity was negativly correlated with apoptosis of breast cancer ( r =-0.67, P <0.01). Conclusion The intensity of telomerase activity and cellular apoptosis are correlated with the size, grade, stage, lymph node number and hormone receptor state of breast cancer. The detection of telomerase activity and apoptosis are helpful in clinical analysis, and may become potential prognostic factor in breast cancer.