P44/42 MAPK和STAT3在γ射线诱发的小鼠白血病骨髓细胞中的表达

The Function of P44/42 Mitogenactivated Protein Kinases and Signal Transducer and Activator of Transcription 3 in Leukemia Marrow Cell Induced by γ-Ray Irradiation on Mice

为了观察P4 4 / 4 2MAPK和STAT3在γ射线诱发的小鼠白血病骨髓细胞中的变化情况 ,首先利用γ射线诱发Balb/C小鼠发生白血病 ,成功地建立了辐射致癌模型 .在此基础上 ,将动物分为三组 :癌变组、辐射未癌变组和对照组 ,利用免疫沉淀和免疫印迹技术 ,检测各组骨髓细胞的P4 4 / 4 2MAPK和STAT3蛋白及磷酸化水平变化情况 .结果显示 :癌变组骨髓细胞P4 4 / 4 2MAPK蛋白及磷酸化水平均高于辐射未癌变组和对照组 (P <0 0 5 ) ;而STAT3蛋白及磷酸化水平在三组骨髓细胞之间无显著差异 (P >0 0 5 ) .说明Ras/P4 4 / 4 2MAPK途径可能在γ射线诱发的小鼠白血病中发挥一定的作用 。

In order to study the possible role of the p44/42 mitogenactivated protein kinases(MAPK) and the signal transducer and activator of transcription 3 (STAT3) in the cancerization process of leukemia marrow cell induced by γ ray irradiation, the mice were divided into three groups according to the pathological examination: the carcinomatous group, the uncarcinomatous control group and the unirradiated control group. The level of phospho STAT3 were detected by the immunoprecipitation and Western blotting assay, and the change of the expression of the P44/42 MAPK, phospho P44/42 MAPK and STAT3 were detected by Western blotting analysis. The results showed that the levels of P44/42 MAPK and phospho P44/42 MAPK were significantly higher in the marrow cell of the carcinomatous group than that in the control groups ( P <0 05) respectively. But statistically, there was no difference in the levels of STAT3 and phospho STAT3 among the three groups ( P >0 05). All the results suggest that there is a possible involvement of Ras/ P44/42 MAPK pathways in the cancerization process of leukemia cell, while JAK/STAT3 pathway makes no contribution to the process of radiation carcinogenesis.