菟丝子提取物在PC12细胞株中的神经营养因子样活性

Neurotrophic Factor-like Activity of Cuscuta chinensis Lam.Extract in PC12 Cell Line

以常用的PC12细胞株为实验模型 ,考察了菟丝子提取物诱导PC12细胞分化及对相关激酶活性的影响 .发现该提取物在诱导PC12细胞分化的同时 ,可明显提高有丝分裂原激活的蛋白激酶 (MAPK)磷酸化 .MAPK激酶的特异抑制剂PD980 5 9,能够有效地抑制菟丝子提取物诱导PC12细胞中MAPK的磷酸化和突起的延伸 ,表明该提取物诱导PC12细胞分化可能与MAPK途径有关 .同时还发现 ,该提取物能一定程度地抑制去血清引起的细胞凋亡 。

Cuscuta chinensis Lam., one of the most important traditional Chinese medicines for tonifying liver and kidney, enhances mitogen-activated protein kinases (MAPKs) activity and as a consequence induces neurite outgrowth in PC12 cells. The effect of Cuscuta chinensis Lam. extract on neurite outgrowth and its potentriated activation of MAPK were similar to that of nerve growth factor. PD98059, a specific inhibitor of MAPK, inhibited the effect of the extract on the phosphorylation of MAPK, suggesting the extract induce the PC12 cells differentiation linked to the MAPK cascade. Furthermore, the extract prevents apoptosis of PC12 cells caused by serum deprivation, indicating that it has neurotrophic factor-like activity.