鼻咽癌差异表达基因PROL4特性分析

Characterization Analysis of Nasopharyngeal Carcinoma Differentially Expressed Gene PROL4

在正常成人鼻咽与鼻咽癌活检组织之间进行抑制性消减杂交和微阵列 (microarray)杂交 ,获得了鼻咽癌差异表达基因PROL4的全长cDNA序列 ,其GenBank登录号为 :AF5 30 4 72 .该基因包含 5 6 7个核苷酸 ,其编码产物是由 134个氨基酸组成的富含脯氨酸蛋白 .采用RT PCR证实了PROL4基因在鼻咽癌细胞株HNE1和鼻咽癌活检组织中表达下调或缺失 (42 /48) .12种组织RNA印迹显示 :PROL4基因在人骨骼肌、胸腺和肺组织中表达 ,其转录本大小约为 0 6kb ,与所克隆的PROL4基因的cDNA大小一致 .进而通过肿瘤表达谱阵列(cancerprofilingarray)杂交检测了其在乳腺癌、子宫癌、结肠癌、胃癌、卵巢癌、肺癌、肾癌、直肠癌、甲状腺癌、子宫颈癌、前列腺癌、胰腺癌、小肠癌组织及其配对的正常组织的表达状况 .

The full-length cDNA sequence of a novel gene PROL4 was obtained through suppression subtraction hybridization and cDNA microarray technique between NPC biopsies and normal adult nasopharyngeal epithelial tissue. PROL4 gene whose GenBank accession number was AF530472 consisted of 567 bp and coding 134 amino acids. RT-PCR confirmed that PROL4 gene was down-expressed in NPC cell line HNE1 and NPC biopsies (42/48). As it was shown by Northern blot, PROL4 gene was expressed in skeletal muscle, thymus and lung, whose transcription size was about 0.6 kb. The expression profiling was further tested by Cancer Profiling Array hybridization in multiple cancer tissues such as breast carcinoma, uterus carcinoma, colon carcinoma, stomach carcinoma, ovary carcinoma, lung carcinoma, kidney carcinoma, rectum carcinoma, thyroid carcinoma, cervix carcinoma, prostate carcinoma, pancreas carcinoma and small intestine carcinoma.