摘要
人N 甲基 D 门冬氨酸受体 (NMDAR ,NR)单克隆抗体MABN1具有明确的抗兴奋毒保护作用 ,但其机制不明 .以MABN1和MK 80 1分别预处理海马细胞 ,拮抗谷氨酸兴奋毒损伤作用 ,采用傅里叶变换红外光谱(FTIR)技术 ,对不同处理后的海马细胞红外光谱特性进行比较 .将去卷积的酰胺Ⅰ带进行曲线拟合后发现 ,MABN1组与MK 80 1组的蛋白质二级结构有明显不同 。
The monoclonal antibody MABN1 against NMDAR could protect the neurons from excitotoxicity, but the mechanism is unknown. Cultured hippocampus neurons treated with glutamate or/and NMDAR antagonist (MABN1 and MK 801) were studied by FTIR spectroscopy. Spectroscopic differences were observed between MABN1 treated and MK 801 treated samples. Curve fitting of the deconvoluted amideⅠband revealed the difference of protein second structure between MABN1 protected neurons and glutamate treated neurons, by which it was presumed that MABN1 protects neurons against excitotoxicity by the mechanism different from the one of MK 801.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第2期290-294,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目 (3 0 0 70 2 67)
中国博士后科学基金(2 0 0 1)
辽宁省重点科技攻关课题项目(2 0 0 12 2 60 0 5 )~~
