苹果提取物抑制肝癌细胞系生长的实验性研究

The inhibited activities of apple extracts on cell proliferation of liver can-cer

目的 观察苹果提取物在体外对肝癌细胞系(HepG2)生长的抑制作用。方法 新鲜富士苹果利用丙酮法提取,不 同浓度苹果提取物与HepG2细胞系混合培养,利用MTS法检测提取物对于肿瘤细胞生长的抑制程度。结果 富士苹果提取物对于HepG2细胞生长有一定程度的抑制作用,在50mg/ml时,抑制率达39.03％。结论 苹果提取物在体外具有抑制肝癌细胞系生长的作用。

Objective To observe the inhibited activities of apple extracts on liver cancer cell lines(HepG2) in vitro. Methods Fresh Fuji apple were extracted using acetone and the extracts were co-cultured with HepG2 cells, effects of Fuji apple extracts on cancer cell proliferation were examined by MTS assay. Results Apple extracts tested had a greater inhibitory effect on HepG2 cell proliferation, Fuji apple inhibited cell proliferation by 39.03% at a dose of 50mg/ml. Conclusions Apple extracts tested have the potential to inhibit the growth of liver cell lines.