Toremifene诱导MCF-7/ADR细胞凋亡的研究

Toremifene Induced Apoptosis in MCF-7/ADR Cells

目的 研究Toremifene体外诱导乳腺癌耐药细胞MCF-7/ADR凋亡和凋亡相关基因Fas、FasL、p53和Caspase-3表达的关系。方法 以不同浓度Toremifene(0.24μg/ml、2.4μg/ml、24μg/ml)诱导MCF-7/ADR细胞凋亡;以MTT法观察不同处理浓度的Toremifene对MCF-7/ADR细胞DNA合成活性的影响;以免疫组化检测Fas、FasL、p53和Caspase-3蛋白表达。结果 不同浓度Toremifene处理的MCF-7/ADR细胞出现DNA合成活性下降,抑制强度与浓度有关(P<0.05);Fas、FasL、p53和Caspase-3蛋白呈上调表达。结论 Toremifene能诱导细胞凋亡;增加Fas、FasL、p53和Caspase-3基因表达;并且与启动Caspase-3凋亡信号和p53直接介导的凋亡有关。

Objective To study the rektionship between apoptosis of MCF-7/ADR cells induced by Toremifene and the expression of Fas/FasL、P53 and Caspaae- 3 genes. Methods Different doses (0.24μg/ml、2.4μg/ml、24μg/ml) Toremifene were used to induce the apoptosis of MCF-7/ADR cells; MTT method was used for detecting DNA synthesizing activity. The expression of Fas/FasL、p53 and Caspase-3 protein was detected by immunohistochemical method. Results The MCF-7/ADR cells treated by different doses Toremifene demonstrated prominent morphologcial features and retardation of cellular growth accordingly, whose IR{ inhibiting rate) had relationship with doses ( P < 0.05) . The expression of Fas/FasL、 p53 and Caspase-3 protein was up-regulated. Conclusion Toremifene not only can induce the apoptosis of MCF-7/ADR cells and enhance the Fas/FasL、p53 and Caspase-3 genes but also is related to the start of conducting Caspase apoptosis message and p53 induced apoptosis.