肠易激综合征患者细胞免疫状态及其临床意义

Level of T-lymphocyte sub-populations in patients with irritable bowel syndrome

目的:探讨肠易激综合征(IBS)患者细胞免疫改变及其在发病机制中的作用。方法:将IBS发作期患者61例按临床主症分为腹泻型、便秘型、腹泻便秘交替型3组,同时设立正常对照组20例。采用流式细胞仪测定外周血T细胞亚群分布百分率(CD3+、CD4+、CD8+、CD4/CD8)。结果:IBS患者外周血T细胞亚群分布百分率异常,特别是辅助性T细胞亚群(CD4)百分率下降和CD4/CD8比例失调明显(P<0.01):腹泻型组和交替型组比值下降;便秘型组比值增高(P<0.01)。结论:IBS患者体内存在细胞免疫网络的调控失常,且各型患者的基础免疫状态各不相同,CD4+细胞均值和CD4/CD8比值可作为判断IBS患者病情进展的指标。

Objective:To investigate the role of malfunctioning immune system in the patho-genesis of irritable bowel syndrome (IBS)and its relationship to clinical outcome.Methods:Sixty-one patients with IBS were available for evaluation of the function of immune system while20healthy people were selected as controls.The expression of CD3 + ,CD4 + ,CD8 + of T lymphocyte was analyzed by flow cytometry.Results:The percentage of CD4 + ,CD8 + T lymphocyte in IBS distributed abnormally.The ratio of CD4/CD8was lower in the diarrhea predominant IBS(IBS-D)and higher in the consti-pation predominant IBS(IBS-C)than in control group(P<0.01).Conclusion:Our studies indicated that an abnormally actived autoreaction of cellular immunity can be detected in IBS and the CD4 + /CD8 + ra-tio could be an useful diagnostic and prognostic marker for IBS.