生殖器溃疡患者杜克雷氏链杆菌、梅毒螺旋体及人类单纯疱疹病毒2型抗体血清学检测及其与HIV感染的关系

Serosurvey of haemophilus ducreuyi,treponema pallidum and herpes simplex virus type-2 in female genital ulcer diseases and their associations with human immunodeficiency virus infection

目的:检测女性生殖器溃疡患者血清杜克雷氏链杆菌抗体、梅毒螺旋体血清学、人类单纯疱疹病毒2型抗体,并探讨其与人类免疫缺陷病毒(HIV)感染之间的关系。方法:将102例女性生殖器溃疡患者作为研究对象。采用酶联免疫吸附法(ELISA)测定血清HIV抗体,并以蛋白印迹试验(westernblot)进行确认;采用快速血浆反应试验(RPR)筛查梅毒,对螺旋体感染患者进一步进行梅毒螺旋体红细胞凝集试验(TPHA);采用Westernblot测定人类单纯疱疹2型特异糖蛋白抗体;采用酶免法(EIA)测定抗杜克雷氏链杆菌抗体IgA及IgG。结果:杜克雷氏链杆菌IgG或/及IgA阳性共计30例(30.61%);梅毒RPR或/及TPHA阳性51例(52.04%);单纯疱疹2型特异糖蛋白抗体阳性17例(17.35%)。98例患者中,HIV抗体阳性47例(47.96%)。30例软下疳患者中19例HIV抗体阳性(63.33%),51例梅毒病例中21例(41.18%)呈HIV感染,17例单纯疱疹病毒2型特异糖蛋白抗体阳性者中7例(41.18%)HIV抗体阳性;三者之间HIV抗体阳性率无显著差异,但均有易感倾向。结论:杜克雷氏链杆菌、梅素螺旋体及单纯疱疹2型感染所致的女性生殖器溃疡与HIV感染有密切联系。

Objective:To investigate seroprevalence of haemophilus ducreyi(chancroid),Tre-ponema pallidum(Syphilis),and herpes simples virus type-2(HSV-2)in patients with genital ulcer dis-eases(GUDs ),and determine their associations with human immunodeficiency virus(HIV)antibody sta-tus.Methods :One hundred and two GUDs cases were enrolled into present study.HIV infection was detected by ELISA and confirmed by Western blot.Syphilis serology was measured by the nontrepone-mal Rapid Plasma Reagin test(RPR)and confirmed by T.Pallidum hemagglutination assay(TPHA).Reactivity against HSV-2-type-specific glycoproteins was assessed by Western blot techniques.Anti-H.ducreyi IgG and IgA antibodies was standardized by enzyme immunoassay(EIA).Results:The positive cases of H.ducleyi IgG,IgA antibodies and both of them were11,13and6respectively,the overall prevalence was30.61%.Four cases were reactive for RPR only,and47patients were reactive in bothRPR and TPHA test,it showed52.04%syphilis seropositivity.There were17cases(17.35%)of posi-tive HSV-2antibodies of98cases of GUDs,the overall prevalence of HIV was47.96%(47cases).Nineteen patients(79.17%)out of24cases of chancroid were HIV seropositive.In51cases of syphilis,there were21HIV seropositive.The seroprevalence of HIV in positive cases of HSV-2antibodies was41.18%(7/17).The HIV antibody status in three above GUDs was not significantly different (P>0.05).Conclusion:GUDs caused by H.ducreyi,T.Pallidum,and HSV-2were significantly more likely to be HIV seropositive.