低分子肝素明胶微球的研制及其口服吸收效果观察

Preparation of low melecular weight heparin gelatin microsphere and its oral absorption

目的:研制低分子肝素口服明胶微球并观察其口服吸收效果。方法:用乳化凝聚法制备低分子肝素明胶微球,以正交实验设计确定最佳制备工艺,用光学显微镜观察其形态及粒径分布,以大鼠用药前后血液凝固时间的变化研究其口服吸收的效果。结果:低分子肝素明胶微球为圆形或椭圆形,粒径范围0.5～20μm,包封率89.4%;大鼠用药后血液凝固时间显著延长。结论:明胶微球载体对低分子肝素大鼠口服吸收有良好的促进作用。

Objective:To prepare gelatin microsphere of low molecular weight heparin and study its oral absorption in rats.Methods:Gelatin microspheres were prepared through the way of emulsification and agglomeration,and their shape and diameter distribution were observed under the light microscope.The optimal technique was chosen with orthogonal design.Oral absorption was determined by the changes of rat blood coagulation time.Results:Gelatin micro sphere of low mo lecular weight heparin were globular or elliptic,the diameter scope was betwee n0.5and20μm,and the encapsulated ratio89.4%.Rat blood coagulation time wa s significantly prolonged.Conclusion:Gelatin microsphere has a significant oral absorption enhancing effect on low molecular weight heparin.