雌激素对大鼠隔海马通路损伤后认知能力的影响

Influence of estrogen on the capability of rat cognition after cutting off diazoma-hippocampus circuit

目的:探讨雌激素对隔海马通路损伤后大鼠认知能力的影响。方法:用4月龄雌性SD大鼠36只,其中18只在脑立体定位仪下切断隔海马通路纤维。术后分为正常组、正常用药组、隔海马通路损伤对照组和用药组(各9只大鼠);用药组手术后当天用药(苯甲酸雌二醇肌注,0.2 mg/(kg·d)连续10 d,然后用Morris水迷宫检测大鼠学习能力。结果:定位航行实验取各组后5个潜伏期作统计学处理,正常组:15.3±6.1 sec;正常用药组:10.5±4.3sec;损伤对照组:41.1±16.6 sec;损伤用药组:98.3±19.5 sec,各组间差别均有显著性意义。结论:隔海马通路损伤后大鼠学习记忆能力下降,雌激素治疗不能提高隔海马通路损伤大鼠学习记忆能力,相反,对学习记忆能力有一定的抑制作用;但正常大鼠加用雌激素对学习记忆能力有一定的促进作用。

Objective:To investegate the influence of estrogen on the capability of rat cognition after cutting off diazoma-hippocampus circuit. Methods:Thirty six SD rats (4 months old,female) were randomly divided into 4 groups (n = 9): group A (no-injury),group B (no-injury and with estrogen) ,group C (injury) and group D (injury and with estrogen). Among 36 rats (C and D group), the fiber of diszoma-hippocampus circuit from 18 rats were cutting off with brain solid positioner. Group B and D were taken benzestrofol by i. m. (0.2 mg/kg/day) after finishing the operation,and the same perfomance continuted for 10 days. Rat's cognition was evaluated with Morris water maze. Results: After finishing place navigation,the latter 5 latencies were processed with statistical analysis. The time of group A,B,C,D were 15.3 ± 6.1 seconds, 10.5 ± 4.3 seconds,41.1 ± 16.6 seconds,98.3 ±19.5 seconds respectively. The time between every 2 groups showed significant discrepancy. Conclusion:The capability of learning and memory of rats is damaged after cutting off dia-zoma-hippocampus circuit. Treatment with estrogen restrain rather than improve the capability of learning and memory of injuried rats. However,estrogen can improve the capability of learning and memory of rats of group A (no-injury).