生后大鼠发育不同时期各级卵泡凋亡的观察

Observation of follicle apoptosis of at different stages of rat development

目的:观察研究生后不同阶段大鼠卵巢卵泡的生长发育与卵泡凋亡的关系、凋亡过程及规律,探索性激素血液浓度变化与卵泡凋亡的内在联系。方法:用DNA缺口原位末端标记检测法,对生后20—140天不同阶段大鼠卵巢卵泡的生长发育及卵泡凋亡进行了光镜观察,同时对生后各阶段鼠血液孕激素(progesterone P、卵泡刺激素(follicleslimulating hormone FSH)、黄体生成素(luteinizing hormone LH)、雌二醇(estradiol E2)浓度进行放免检测。结果:生后20天鼠卵泡即可见到凋亡发生,卵泡凋亡始于卵母细胞,继则是与其相邻的卵泡细胞,卵泡细胞的凋亡从内向外依次发生。卵泡的凋亡数目随着卵巢的生长发育而逐渐增多,生后40天可见到黄体细胞凋亡发生,至50—60天可见到卵泡膜内膜细胞凋亡及白体形成,出生80天后卵巢发育已完全成熟,此时卵泡的凋亡数也达到了高峰;放免结果经方差分析显示孕激素P>0.05,差异显著,推测其浓度变化与卵泡凋亡有内在联系。

Objective:To investigate the relationship between follicle development and follicle apoptosis of rat ovaries at different stages, and to find out the relation between the concentration changing of sex hormone in rat blood and follicle apoptosis. Methods: With TUNEL method,light microscope was used to observe the development and apoptosis of ovarian follicles of rat from the 20th to 140th d respectirely after birth, the concentrations of P,FSH,LH and E2 in blood were examined by radioimmunoassay. Results: Apoptosis appeared in rat follicle at the 20th d after birth. It started from oocyte and then followed by adjoining follicular cells. Apoptosis happened regularly from inside to outside. The numbers of follicular apoptcxsis increased gradually along with the growth of the ovaries Apoptosis occured in corpus luteum 40th d afrer birth. Between the 50-60th d apoptosis of inner membrane cells of follicle appeared and something remained as white bodies. The ovaries grew marure 80 d after birth and the numbers of folliculart apopotosis became the largest. By analysis of variance the result of radioimmunassay showed that (P<0.01)the change of progestine concentration was obvious. Conclusion:Follicle apoptosis of rat ovaries can be observed 20 d after birth, then increases progressively and reaches the peak 80 d after birth. The concentration of progestine among sex hormones varies greatly,and it is estimated that the change of progestine concentration is connected with follicle apoptosis.