摘要
从被超声波破碎的猴头菌发酵菌丝细胞热水提取液中,经乙醇分级分离得到单一级分,HPA经SepharoseCL-6B柱层析测得其相对分子质量为4 56×105.经部分酸水解、高碘酸氧化、Smith降解和甲基化分析确定此多糖以(1→4)糖苷键连接,平均每8个残基上有1个分枝,分枝点在O-3上,分枝末端残基为Glc1—.
A ethanol graded componet-HPA was seperated from the boiled exatraction of ultrasonic-broken cells of zymogenic thread of hericium erinaceus.The molecular weight of the HPA is 456 000 measured by sepharose CL-6B.By analyzing the products of partial hydrolysis with acid,periodate oxidation,Smith degradation and methylation,the structure of HPA was determined as 1→4 linked Glc with a Glc1- branch attached to O-3,the branch occurred at the position in an average of glucose residue on backbone.
出处
《分子科学学报》
CAS
CSCD
2003年第2期123-126,共4页
Journal of Molecular Science
基金
国家自然科学基金资助项目(39870847)
