环氧化酶-2在正常成年大鼠睾丸中表达的研究

Cyclooxygenase-2 expression and its localization in the mature rat testis

目的 检测环氧化酶 2 (COX 2 )在大鼠睾丸组织中的表达情况 ,探讨COX 2在机体正常生殖机能中的作用机理。 方法 采用免疫组织化学、RT PCR和Western blot方法检测 5只正常成年大鼠睾丸组织中COX 2的表达。 结果 RT PCR和Western blot方法证实COX 2在基因和蛋白质水平均有表达 ;免疫组织化学结果显示 5只大鼠睾丸组织生精细胞中均有COX 2表达 ,其中初级精母细胞为强阳性 ,COX 2定位于曲细精管内的生精细胞。 结论 正常成年大鼠睾丸的生精细胞中存在COX 2表达 ,COX 2可能与睾丸的精子生成过程有关。

Objective To confirm the expression and localization of COX-2 gene in mature rat testis,as COX-2 not only plays a central role in inflammation by up-regulating the synthesis of prostaglandins,but also mediates critical reproductive functions by other mechanisms. Methods In the mature Wistar rats,COX-2 expression and its localization were examined by using immunohistochemistry,RT-PCR and Western-blot. Results The results of RT-PCR and Western-blot showed that COX-2 mRNA and its protein exist in the testis. Expression of COX-2 was localized in spermatogenic cells on immunohistochemical study.No COX-2 expression in sertoli cells and leydig cells was found. Conclusions The present study confirmed the expression of COX-2 in rat testis and its localization in spermatogenic cells.These suggest that COX-2 may play a role in spermatogenesis.