SARS病人外周血NK细胞和B淋巴细胞的动态变化及发病机理初探

The study of B lymphocyte and NK cell in Severe Acute Respiratory Syndrome (SARS) patients

目的 :初步探讨外周血NK细胞和B细胞在严重急性呼吸综合征 (severeacuterespiratorysyndrome ,SARS)发病进程及发病机理中的意义。方法 :应用流式细胞仪检测和分析SARS病人外周血B淋巴细胞、NK细胞动态变化 ,使用的NK细胞表面标志为CD16 + CD5 6、B细胞表面标志为CD19。结果分析用BD公司的MultiSet自动分析软件。结果 :随着病程的进展92 %的SARS病人B细胞先降后升或持续升高 ,少部分病人B细胞无改变或下降。NK细胞显示不同的个体差异 ,73.7%的病人显示典型的抗病毒免疫现象 ,即发病初期NK细胞数升高 ,恢复期NK细胞数下降 ,也有部分病人NK细胞无变化 (15 .8% )或升高 (10 5 % )。结论 :在SARS发病进程中B淋巴细胞介导的体液免疫可能起一定的作用。

Objective:To study the evolution of B lymphocyte and NK cell in Severe Acute Respiratory Syndrome (SARS) patients.Methods:B lymphocyte and NK cell in the peripheral blood was detected by flow cytometry in 53 patients with SARS.Results:During the disease progression,B lymphocyte decreased then increased or increased progressively in 92% cured SARS patients, in others have no change or decrease.The NK cell showed individual difference: typical immune response was showed in 73.7% patients, but in others have no change or increase.