颊癌诱导淋巴管生成作用的研究

A study of lymphangiogenesis induced by carcinoma of oral buc cal mucosa

目的 :初步研究颊癌诱导淋巴管生成作用 ,探讨其与癌淋巴路转移间的相关关系。方法 :原位杂交和免疫组化方法研究内皮细胞生长因子VEGF C及其受体Flt 4在 5 6例口腔颊癌原发灶组织中的表达情况 ,并统计分析VEGF C表达与癌浸润方式、淋巴管侵入及淋巴结转移等临床病理表现之间的相关关系。结果 :颊癌细胞表达VEGF C与癌间质淋巴管表达Flt 4显著相关 (P <0 0 0 1) ,且癌细胞侵入淋巴管及淋巴结转移病例VEGF C阳性表达率显著高于无转移或未侵入病例 (P <0 0 0 1)。结论 :颊癌表达VEGF C和诱导淋巴管生成可能是发生淋巴路转移的重要条件 。

Objective:To investigate lymphangiogenesis in d uced by carcinoma of buccal mucosa and study the relationships between it and ly mphatic metastasis Methods:The expressions of vascular endothe lial growth factor C and its receptor Flt 4 were investigated in fifty six pr imary squamous cell carcinomas of buccal mucosa by using in situhybridiz ation and immunohistochemical staining Relationships were analyzed statistical ly between VEGF C expression and cancer invasive pattern, lymphatics invasion, lymph nodes metastases, et al . Results:The expressions of VE GF C of cancer cells are significantly correlated to the expressions of Flt 4 of lymphatic endothelial in cancer stroma(P<0 001) Carcinomas with lymphatic s invasion and lymph nodes metastasis were VEGF C positive in significantly hig her rates than those without invasion and metastasis( P <0 001) Con clusion:Expression of VEGF C and induction of lymphangiogenesis of bucc al cancer might be important for lymphatic metastasis and could be a reference t o clinical treatments of neck lymph nodes