中国棉铃虫核型多角体病毒VHA_(273)原毒株及其克隆株的比较研究

Comparison of Heliothis armigera NPV VHA273 Wild Isolate with Its Clone

本文从形态结构、生物活性、核酸限制性内切酶图谱、结构多肽等方面对中国棉铃虫核型多角体病毒(HaNPV)VHA273多粒包埋型原毒株及其单粒包埋型克隆株H9进行了比较研究.它们对中国棉铃虫三龄初幼虫的LC5o值分别为2.987×104PIBs/mL和1.647×104PIBs/mL当感染剂量为2×107PIBs/mL时,其LT5o值分别是4.866d和4.797d.两个毒株的生物活性差别不大.经SDS-PAGE分析,两毒株结构多肽图谱带型相差较大.两毒株基因组经EcoR Ⅰ,BamH Ⅰ,Hind Ⅲ和Xba Ⅰ消化后,得到的内切酶图谱表现为两毒株间有差别.这些差异的发现将有助于从分子水平揭示多粒包埋病毒和单粒包埋病毒形成原因.

Wild HaNPV-VHA_(273) Multi-nucleocapsid NPV and its clone Single-nucleiocapsid NPV H_9 were compared on the shape and structure, biological activity, restriction pattern and structural polypeptide.The LC_(50) values of the wild and clone isolates for early third instar H.armigera larvae were 2.987×10~4 PIBs/mL and 1.647×10~4 PIBs/mL respectively; The LT_(50) values for 2×10~7 PIBs/mL concentration were 4.866 and 4.797 days respectively. Bioactivities of two isolates were similar. Assayed by SDS-PAGE, there were more difference between VHA273 and H_9 structural polypeptides.Digested by EcoRI, Bam HI, Hind Ⅲ and Xba Ⅰ, VHA_(273) and H_9 genomes had some differences in their fragmentation profiles. This discovery will be helpful for revealing the factor what caused the Multi-nucleocapsid NPV and Single-nucleocapsid NPV's formed in molecular level.