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鸡马立克氏病病毒糖蛋白gB基因的表达及基因免疫 被引量:1

Expression and Genic Immunization of Glycoprotein gB Gene of Marek's disease virus
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摘要 将马立克氏病病毒(Marek's disease virus,MDV)gB基因插入真核表达载体pcDNA3中,经酶切和PCR鉴定为正向插入了gB基因的重组质粒,命名为pcDNA3-gB。体外转染COS-7细胞,经间接免疫荧光染色证实转染pcDNA3-gB后的COS-7细胞内有糖蛋白B的表达。用pcDNA3-gB对AA肉用雏鸡腿部肌肉注射,进行一免。间隔2周后,用pcDNA3-gB再加强免疫一次。之后2周攻毒,观察免疫保护效果。结果表明,MDV gB基因DNA免疫可以诱导鸡体产生免疫保护,免疫保护指数为72.2%。 gB gene of Marek's disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.
出处 《中国病毒学》 CSCD 2003年第3期275-278,共4页 Virologica Sinica
关键词 鸡马立克氏病病毒 糖蛋白 GB基因 表达 基因免疫 Marek's disease virus(MDV) gB gene Expression DNA vaccine
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