对虾白斑综合症病毒与虾鳃细胞膜特异性结合关系的确立

Verifying the Specific Binding Between WSSV and Shrimp Gill Cell Membrane

对虾白斑综合症病素养(White spot syndrome virus,WSSV)是养殖对虾的一个主要病原,也是目前发现的基因组最大的动物病素养(基因组约290kDa,双链环状).

Cell membrane extracted from shrimp gill was coated on 96 well plate and bound with DIG labeled White spot syndrome virus (WSSV), then detected with Anti-DIG-Fab-HRP system, meanwhile taking the cell membrane from hepatopancreas, muscle, and E.coli for control group, the result verified the specific binding between WSSV and gill cell membrane. And here the binding assay can be employed as a convenient and rapid way to evaluate the binding nature between WSSV and the shrimp gill cell membrane.