静磁场对大鼠三叉神经节中SP-mRNA作用的实验研究

The Study of Effects of Static Magnetic Field on SP-mRNA in Trigeminal Ganglion in Rats

目的 探讨静磁场对大鼠三叉神经节中SP_mRNA的影响。方法 将 4 4只 6～ 7周的大鼠置于静磁场中 ,在 1、2、6、1 2和 2 4h时分别将动物处死 ,取其三叉神经节 ,用原位杂交方法研究SP_mRNA在各时间点的变化。结果 在静磁场作用下三叉神经节中SP_mRNA的表达呈上调变化 ,2h时达峰值 ,各时间点与正常对照组间均存在显著性差异 (P <0 0 1 )。

Objective\ To evaluate the effect of static magnetic field on the expression of SP_mRNA in TG in rats.Methods\ 44 Wistar rats aged 6_7 weeks were put into static magnetic field and were sacrificed at 1 h, 2 h, 6 h, 12 h, 24 h, respectively. In situ hybridization method was used to evaluate the changes of SP_mRNA expression at different time point.Results\ Many neurons in TG were marked with SP probes in each group, the expression of SP_mRNA increased remarkably in static magnetic field group. In this group, the percentage of SP_mRNA positive neurons in TG increased greatly in 1 h, reached its peak in 2 h, from then on, decrease of the percentage started slowly but a moderate percentage was kept until 24 h, which was thought to be enough to maintain orthodontic tooth movement. The tendency of control group was almost the same with that of experimental group. The expression of SP_mRNA was higher in experimental group within 2 h but became lower after 2 h as compared with control group, this indicated that magnetic field reduced the SP_mRNA expression and exerted restoring effect on trauma. There were significant differences between experimental groups and control group at different time points (P<0 01).Conclusion\ The expression of SP_mRNA in TG in rats increased significantly in static magnetic field.\;