卡介苗胞壁蛋白诱导THP-1细胞IL-12mRNA表达

Enhancement of IL-12 mRNA expression in THP-1 cells induced by BCG cell wall protein

目的 :研究卡介苗 (BCG)能否诱导人单核白细胞系细胞THP 1、IL 1 2P40基因表达 ,并鉴定其胞壁蛋白的活性组份。方法 :应用逆转录聚合酶链反应 (RT PCR)法检测THP 1细胞IL 1 2P40mRNA的表达 ;采用超声破碎细胞、蔗糖密度梯度离心、SephadexG 1 5 0柱层析等方法分离卡介苗胞壁蛋白质组份。 结果 :卡介苗胞壁蛋白SephadexG 1 5 0柱层析所得组分中 ,分子量范围约 2 1～ 2 9kD的组份诱导THP 1细胞IL 1 2P40mR NA的表达增强。结论 :结果显示BCG胞壁蛋白可刺激白细胞IL 1 2P40基因的表达。

Abstact Objection: To study the BCG cell wall proteins that stimulate IL 12 gene expression in human THP 1 cells. Methods: Using reverse transcription PCR (RT PCR) , IL 12 mRNA expression in THP 1 cells was detected. Results: The induced expression of IL 12p40 mRNA was dose and time dependent. SDS PAGE showed its molecular range of a 21 29KD. Conclusion: These results provided that BCG cell wall proteins could stimulate IL 12p40 mRNA expression in human THP 1 cells.