运用AFLP技术估计毛白杨及其杂种毛新杨的遗传杂合水平

ESTIMATION OF THE GENETIC HETEROZYGOSITY OF POPULUS TOMENTOSA AND ITS HYBRID P. TOMENTOSA×P. BOLLEANA USING AFLP TECHNIQUE

首次报道以毛新杨 (Populustomentosa×P .bolleana)无性系TB0 1×毛白杨 (P .tomentosa)无性系LM5 0的回交子代 12 0株随机个体为材料 ,利用扩增性片断长度多态性 (AFLPs)标记技术研究毛白杨及其杂种毛新杨的遗传平均杂合水平。研究结果表明 :30对不同的AFLP引物组合能够检测到毛白杨的遗传平均杂合水平范围为 9 10 %～ 30 19% ,平均为 18 71% ;而毛新杨的遗传平均杂合范围为 1 39%～ 2 0 31% ,平均杂和水平约为 8 4 7%。因此 ,对于分离群体亲本杂合度来说 ,毛白杨平均杂合度为毛新杨的 2 2 1倍。

The average heterozygosity of male clone “LM 50” (Populus tomentosa) and its hybrid female clone “TB01” (P. tomentosa×P. bolleana) was estimated, using AFLP (Amplified Fragment Length Polymorphisms) technique, based on random samples with 120 progeny. The hybrids were derived from an interspecific backcross between the female hybrid clone “TB01” and the male clone “LM50”. The result showed that the average heterozygosity was 18 71% with a range of 9 10% ～ 30 19% for male clone “LM50” ( P. tomentosa) , and 8 47% varied from 1 39% to 20 31% for female clone “TB01” ( P. tomentosa×P. bolleana), respectively, using 30 different AFLP primer combinations. Therefore, the average heterozygosity of clone “LM50” was 2 21 times larger than the one of clone “TB01”, based on the segregation population.