摘要
不同树种近缘种的种子和苗木的真实性鉴别是林木种苗检验过程中常常遇到的难题。本文对国内外林木种苗的真实性鉴别研究及相关信息进行了综述 ,并在此基础上 ,对利用种特异分子标记鉴别林木种苗真实性的技术和方法进行了探讨 :根据在进化上有同源性的突变基因位点或DNA区段可发展种特异的SNP标记 ;而利用物种在进化过程中固定的特异基因位点或DNA区段可发展种特异的STS或SCAR标记。这 2种类型的种特异分子标记均可直接应用到实际的检验过程中。
The authentic identification of seed and seedling in the closely related tree species is a very difficult problem in the forest tree seed and seedling of inspection. The research proceeding and the related information in this area are reviewed in this paper.On these bases,the authores discuss how to develop species\|specific molecular markers to distinguish closely related tree species:one method is to develop species\|specific SNPs markers according to the difference of the homogenous genes or loci in different tree species,the other is to develop species\|specific STS or SCAR markers due to the specific genes or loci fixed in different tree species in the evolution progress.The species\|specific molecular markers developed by these two methods can be used directly in forest tree seed inspection.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2003年第3期129-135,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金 ( 3 0 0 0 0 13 3 )
The International Science Foundation support to Dr Yin (D1997 2 )
