钛合金微粒对硬膜外瘢痕粘连形成影响的实验研究

Experimental Study on the Effect of Titanium Alloy Particles on Epidural Scar Formation

目的 :观察钛合金微粒对硬膜外瘢痕粘连的影响 :方法 :3 6只成年新西兰兔随机分为 3组。切除L5椎板后造成 10mm× 5mm硬膜裸露区 ,A组植入钛合金微粒 ,B组植入不锈钢微粒 ,C组作为空白对照 ,术后 4、8、12周分别进行肉眼和光镜观察 ,8周时进行硬膜外粘连等级评分和计算机图像分析。结果 :4周时 3组均有大量瘢痕形成 ,瘢痕尚未成熟 ,其间可见纤维母细胞。 8周时瘢痕成熟 ,肉眼及光镜硬膜外粘连等级评分 ,三组间无显著差异 (P >0 .0 5 ) ,计算机图像分析示A组、C组的粘连程度均轻于B组 (P <0 .0 1) ,而A组与C组无显著差异 (P >0 .0 5 )。 12周时瘢痕组织转化为骨组织 ,硬膜外大量新生椎板形成 ,呈松质骨状。结论 :钛合金微粒对于硬脊膜及硬膜外组织具有良好的生物相容性 ,其对硬膜外瘢痕粘连的形成及发展无影响。

Objective: To study the effect of titanium alloy particles on epidural scar formation. Method: Laminectomy was performed in L 5 in 36 rabbits which were divided into 3 groups. 10mm×5mm dura mater was exposed. Titanium alloy particles were put on the dura mater of Group A, stainless steel particles were put in Group B, Group C was a control group without any metal particles. The epidural tissues were observed grossly and histologically at 4?8?12 weeks postoperatively. Classification and computed imaging analysis of epidural adhesion and scar formation were done at 8 weeks. Result: Obvious epidural adhesion was formed in all groups at 4 weeks. The scar tissue was not mature and some fibroblasts could be seen. At 8 weeks, the scar tissue became mature and there was no obvious difference on the score of epidural adhesion grossly and histologically among three groups ( P >0.05). But the computed imaging analysis showed that the epidural adhesion in Group B was more serious than that in Group A and C ( P <0.01). And there was no obvious difference between Group A and C ( P >0.05). At 12 weeks, the scar tissues were ossified and new vertebral laminae were formed. Conclusion: The titanium alloy has good biocompatibility with epidural tissues and its particles have no effect on epidural adhesion and scar formation.