食管鳞状细胞癌p16基因变异及其与肿瘤病理的关系

Relationship of p16 Gene Deletion or Mutation and the Clinicopathologic Significance in Esophageal Squamous Cell Carcinomas

目的 探讨 p16基因与原发性食管鳞状细胞癌发生、发展的关系。 方法 应用聚合酶链反应 -单链构象多态分析方法检测 5 3例食管鳞状细胞癌组织中 p16基因的缺失和突变 ,分析 p16基因变异与食管鳞状细胞癌的临床病理关系。 结果 5 3例食管癌患者中 ,14例为 p16基因纯合性缺失 ,12例存在突变。p16基因变异在淋巴结转移、远处转移及临床分期方面差异有显著性 (P<0 .0 5 ) ;在性别、年龄、肿瘤长度及肿瘤浸润深度方面差异无显著性 (P>0 .0 5 )。 结论 p16基因的缺失与突变可能是食管鳞状细胞癌的晚期事件 ,它在食管癌的发生、发展中起重要作用。p16基因缺失与突变的检测可作为判断食管鳞状细胞癌恶性程度。

Objective To explore the relationship of p16 gene deletion or mutation and the tumorigenesis as well as the development of primary esophageal squamous cell carcinomas. Methods 53 samples from patients with esophageal squamous cell carcinoma were enrolled. The mutation of p16 gene was detected by polymerase chain reaction and single strand conformation polymorphism(PCR\|SSCP). Results 14 deletions and 12 mutations had been detected. Between wild and mutant p16 groups, there were significant differences in lymph node metastasis, distance metastasis and clinical stage(P<0 05), but no significant differences in sex, age, length of tumor, invasion's depth of tumor(P>0 05). \{Conclusion\} The mutation of p16 gene may be the late event in esophageal squamous cell carcinoma and it plays an important role in the tumorigenesis and development of esophageal squamous cell carcinoma. Detecting the mutation of p16 gene may serve as a marker of predicting degree of maligmancy, development and prognosis of esophageal squamous cell carcinoma.