成釉细胞瘤中Cyclin D1和CDK4表达的定量分析

Quantitative study on expression of Cyclin D1 and CDK4 in ameloblastoma

目的 检测成釉细胞瘤中Cyclin D1和CDK4的表达,探讨其在成釉细胞瘤中的作用及意义。方法 选取52例成釉细胞瘤,其中良性35例,恶性17例,另取10例正常口腔黏膜作对照研究。应用免疫组化SP方法和原位杂交技术检测52例成釉细胞瘤和10例对照的正常口腔黏膜Cyclin D1蛋白及其mRNA和CDK4蛋白的表达。结果 良性和恶性成釉细胞中Cyclin D1蛋白及其mRNA的表达均显著高于对照的口腔黏膜(p<0/01)。CDK4的表达在良性、恶性和对照的口腔黏膜各组之间均具有显著性差异(p<0.01)。复发组CDK4的阳性细胞率(76.26±20.36)显著高于未复发组(65.45±17.41)(p<0.01)。在性别、年龄、发病部位、瘤体大小等方面Cyclin D1和CDK4表达均无显著性差异(p>0.05)。Cyclin D1蛋白及其mRNA的表达呈显著的正相关关系(p<0.01)。结论 Cyclin D1和CDK4参与成釉细胞瘤的发生和发展,CDK4可作为判断预后的参考指标。

Objective The expression of cyclin D1 and CDK4 was detected to determine the role in the initiation and developement in ameloblastoma. Method 52 cases of ameloblastoma(AB) were chosen including benign AB (35 cases) and malignant AB (17 cases). 10 cases of normal oral mucosa were studied as contral group. The expression of cyclin D1 and CDK4 was examined immunohistochemically using SP method and the expression of cyclin D1 mRNA was investigated by in situ hybridization technique. Result The expression of cyclin D1 and mRNA in benign or malignant AB was significantly higher than that in normal oral mucosa(p<0.01). The expression of CDK4 showed a significantly difference betweent each type of benign, malignant AB and normal oral mucosa(p<0.01). The positive cell ratio of CDK4 in recurrence group was significantly higher than that in no recurrence group(p<0.01). There existed no! difference about expression of cyclin D1 and CDK4 in sex, age, location and tumour size parameters(p>0.05). The exqression of cyclin D1 protein demonstrated a positive correlation with cyclin D1 mRNA(p<0.01). Conclusion Cyclin D1 and CDK4 may be concerned with the initiation and progression in ameloblastoma. CDK4 may be helpful in evaluating the prognosis in ameloblastoma.