果蔬中维生素C的形态分析研究

Determination of vitamin C in vegetables with fluorescence spectrophotometry

采用荧光分光光度法进行了果蔬中维生素C的形态分析研究.结果表明:在HPO3 HAc介质中,抗坏血酸(AA)能被2,6 二氯酚靛酚(DCP)氧化为脱氢抗坏血酸(DAA),DAA与邻苯二胺(OPDA)反应生成荧光性物质,在一定条件下,方法的线性范围为0～100μg/15mL,精密度(RSD)为1%,回收率为97%～105%.

Formation analysis of vitamin C with the fluorescence spectrophotometry method was studied.In HPO3HAc solution,ascorbic acid(AA) can be oxidized by 2,6Dichloriondophenol(DCP) into dehydroascorbic acid (DAA),the DAA reacts with ophenylenediamine(OPDA),the resultant is epipolic.The linear range of ascorbic acid is 0～100 μg/15 mL,the relative standard deviation(RSD) is 1%,and the recovery rate is 97%～105%.