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设计表达兔防御素基因NP1

Design and Expression of Rabbit Gene Defensin NP1
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摘要 利用计算机软件对在将大肠杆菌中表达的兔防御素NP -1基因进行分析。消除宿主菌不表达或低效表达的密码子。同时对NP -1基因与质粒 pGEX -4T -1中融合基因GST进行RNA二级结构预测 ,通过对RNA二级结构进行分析 ,消除可能影响基因表达的二级结构 ,设计了有利于今后对目的基因的表达的兔防御素基因 ,并在大肠杆菌高效表达。结果表明 :合成基因可以在大肠杆菌中高效表达。 The rabbit defensin NP-1 gene which will be expressed in E.coli was analyzed by software.The codon with little no expression(was eliminated).At the same time,the RNA and TIR secondary structure of GST gene and GST-defensin fusion gene in pGEX-4t-1 vector was pridicted,and the secondary structure which will affect the expression was eliminated.
作者 徐文生 许杨
机构地区 江西-OAI联合研究院
出处 《江西农业大学学报》 CAS CSCD 2003年第3期418-422,共5页 Acta Agriculturae Universitatis Jiangxiensis
关键词 兔防御素基因NPl 融合基因 质粒 pGEx表达 secondary structure fuse gene vector pGEX expression
分类号 Q786 [生物学—分子生物学]
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江西农业大学学报
2003年 第3期

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