肾综合征出血热山东分离株鲁宁-84刘株的分子特征分析

Molecular characterization of hantavirus Shandong isolate JNL virus strain

目的 分析肾综合征出血热山东分离株鲁宁 84刘株的分子特征。方法 应用逆转录聚合酶链反应 (RT PCR)扩增鲁宁 84刘株M和S片段全基因 ,克隆于T载体 ,纯化后测定序列 ,应用DNASTAR软件比较分析。结果 鲁宁 84刘株 (JNL株 )的M片段的全基因序列共 36 15个核苷酸 ,编码 1135个氨基酸 ,S片段的全基因序列共 16 98个核苷酸 ,编码 4 2 9个氨基酸 ,为HTN型毒株。JNL株与HTN型毒株M和S全基因序列差异分别高达 2 0 0 %～ 2 0 6 %和 15 5 %～ 16 0 % ,基于核苷酸序列的种系发生分析结果也可以看到 ,鲁宁 84刘株不同于国内外其他的分离株 ,处于独立的分支。结论 山东地区鲁宁 84刘株流行株是与国内外HTN型毒株不同的新的HTN亚型病毒。

Objective To understnad the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain Methods The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT\|PCR,and the purified PCR product was cloned into T vector for sequencing Results The results revealed that the JNL M segment was 3 615 bp in length,encoding 1 135 amino acids,and the S segment was 1 698 bp encoding 429 amino acids,JNL belongs to HTN virus The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20\^0% 20\^6% and 15\^5% 16 0% respectively Phylogenetic tree also showed that the position of JNL is located at a different clade Conclusion HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus