大黄乙醇提取物体内抗单纯疱疹病毒作用的研究

Inhibition effects of rhubarb ethanol extract on herpes simplex virus infection in vivo

目的 了解大黄乙醇提取物 (大黄 )体内抗单纯疱疹病毒 (HSV)的作用。方法 小鼠尾静脉接种滴度为 10 3TCID50 HSV Ⅰ型 0 15ml,第 2天皮下注射给药。将BALB c小鼠分为 7个组 ,分别给予不同剂量的药物 ,不同时间取肝、脾、肾等组织 ,做病理切片 ,观察病变情况 ;用空斑形成法滴定各脏器中的病毒滴度 ,观察药物在动物体内对HSV感染的抑制作用。结果 大黄乙醇提取物经皮下注射未发现小鼠有毒性反应 ;大黄各治疗组脾脏不出现病理变化 ,中、高剂量组肝脏、肾脏的病理损伤也能逐渐消失 ;大黄低剂量组与ACV阳性对照组效果相当 ;病毒滴度测定说明大黄能使各脏器中病毒滴度迅速下降 ,中、高剂量组比低剂量组作用明显 ;将各组数值进行两两比较 (Q检验 ) :F =4 9 14 5 9,P <0 0 0 1,总体均数差异有显著性 ;治疗组 (ACV、DH1、DH2、DH3)与非治疗组 (VC)差异有显著性 (P <0 0 1) ;DH2、DH3、与DH1差异有显著性 (P <0 0 1) ;DH1、DH2、DH3与ACV差异无显著性 (P >0 0 5 )。结果说明 ,对于总滴度平均值的下降 ,大黄与ACV同样有效 ,且中、高剂量组的效果优于低剂量组。结论 大黄乙醇提取物在体内具有非常明显的抗HSV感染作用 ,应用前景广阔。

Objective To know the anti viral effects of rhubarb ethanol extract (REE) on herpes simplex virus(HSV) infection in vivo Methods BALB/c mice inoculated from tail vein with 0 15 ml of HSV Ⅰ (TCID 50 =10 3) were injected hypodermically with REE next day After divided into seven groups, three groups of mice were given different doses of REE respectively and the other groups as controls Pathological sections from the liver, spleen, kidney were made at different times of postinfection, and their pathological changes were observed under microscope; the virus titers in viscera were assayed by using plaque formation technique and the rhubarb inhibitions to the infection of HSV in vivo were observed Results No toxic response to mice were observed for REE injected hypodermically; no pathological changes were observed in different therapy groups of spleens And those in livers and kidneys at medium and high dosed groups disappeared quickly The effect of low dosed group was equal to that of positive control group, acyclovir(ACV); the results of the titer tests showed that the virus decreased rapidly by using REE, especially in the medium and high dosed groups which were much more marked than the low dosed group; Q test of the data showed that total mean value had statistical significance( F =49 1459, P <0 01 ); moreover there were statistical significance between therapy groups ( ACV, DH1,DH2,DH3 ) and non therapy groups (VC) ( P <0 01 ) and between DH2, DH3 and DH1 ( P <0 01 ); no statistical significance were found between DH1, DH2 or DH3 and ACV ( P >0 05) Results show that as to the effect of decreasing the average of the total titer, rhubarb is as effective as ACV; furthermore, the medium and high dosed groups are superior to the low dosed group Conclusion REE has significant anti viral effect on HSV in vivo ; there will be a wide application foreground of it in clinical usage