亚砷酸抗裸鼠人肝癌作用及机制研究

Action and mechanism of arsenious acid to human hepatocarcinoma in nude mouse

目的 探讨亚砷酸体内对人肝癌细胞的作用、毒副作用及机制。方法 人肝癌细胞BEL 74 0 2体外培养 ,取指数生长期细胞裸鼠皮下接种 ,成瘤后腹腔分别连续注射不同浓度亚砷酸、5 Fu及生理盐水 ,观察不同浓度亚砷酸治疗组及对照组瘤块大小、抑癌率和裸鼠一般状态的变化 ,光镜下观察肝肾结构的改变 ,并进行血常规检测。结果 亚砷酸可明显抑制瘤块增长 ,2 .5mg kg亚砷酸 10天的抑瘤率为 5 3.4 2 % ,5mg kg亚砷酸 5天、10天的抑瘤率分别为 79.2 8%、96 5 8% ,而且无明显肝肾损害 ,对造血系统无影响。结论 亚砷酸可抑制体内肝癌结节生长 ,而且对肝。

Objective To study the effect of arsenic trioxide(As 2O 3) on human hepatoma cell line BEL 7402 in vivo. Methods Human hepatoma cell line BEL 7402 cultrued in vitro was inoculated in nude mouse, arsenic trioxide,5 Fu and saline were injected imtraperitoneally into nude mouse,respectively.Studied the changes of the size of tumor and general condition of nude mouse;observed the structural change of liver and kidney. Results As 2O 3 could inhibit the growth of tumor.The tumor growth inhibition rate in mice treated with 2.5 mg/kg As 2O 3 was 53.42% in the tenth day;the tumor growth inhibition rate in mice treated with 5mg/kg As 2O 3 was 79.28% in the fifth day,96.58% in the tenth day,respectively.As 2O 3 could not damage the liver and kidney of nude mouse,and could not affect the system of blood.Conclusion Aresenic trioxide can inhibit the proliferation of tumor.There is no side effect to the liver,kidney and the system of blood.