期刊文献+

三氧化二砷与维甲酸联合作用在NB4,MR2细胞系中的体外研究 被引量:2

Study on the effects of arsenic trioxide and all-trans retinoic acid on NB4 and MR2 cell lines in vitro
下载PDF
导出
摘要 目的 观察三氧化二砷 (As2 O3)和全反式维甲酸 (ATRA)联合作用对NB4 ,MR2不同亚型急性早幼粒白血病细胞株 ,诱导分化、增殖、凋亡的影响。方法 以NB4 ,MR2为体外模型 ,利用细胞生长曲线、台盼蓝拒染法、MTT法、NBT、细胞形态Wrigh’s Gimesa染色 ,观察细胞增殖、分化、凋亡的相互作用。结果 NB4细胞株 :0 .5 μmol LAs2 O3与 10 - 6 mol LATRA对细胞增殖、分化、凋亡呈现拮抗 ,而 0 .5 μmol LAs2 O3与 (10 - 7~ 10 - 8mol L)ATRA呈现协同 ;MR2细胞株 :0 .5 μmol LAs2 O3与 10 - 6 mol LATRA对细胞增殖、分化、凋亡呈现协同。结论 As2 O3与全反式维甲酸协同效应呈现不同细胞、剂量的特异性 ,MR2细胞株协同效应明显强于NB4细胞株。 Objective To investigate the effects of arsenic trioxide and all trans retinoic acid on the inducement differentiation, proliferation, apoptosis of NB4,MR2 cell lines of acute promyelocytic leukemia. Methods To observe proliferation, apoptosis and differentiation,methods of cell growth curve, trypan blue exclusion assay, MTT, NBT reduction, and Wrigh's Gimesa stain were used. Results NB4 cell lines: 0.5μmol/L As 2O 3 conteracted with 10 -6 mol/L ATRA on cell proliferation, differentiation, apoptosis; but 0.5μmol/L As 2O 3 and (10 -7 ~10 -8 mol/L )ATRA showed synergy effects; MR2 cell lines: 0.5μmol/L As 2O 3 and 10 -6 mol/L ATRA showed synergy effects. Conclusion The synergy effect of As 2O 3 and all trans retinoic acid take on specificities on different cells and doses, and the effect on MR2 cell lines is much stronger than on NB4 cell line.
出处 《哈尔滨医科大学学报》 CAS 2003年第3期218-222,共5页 Journal of Harbin Medical University
关键词 三氧化二砷 维甲酸 联合作用 NB4细胞系 MR2细胞系 体外研究 急性白血病 arsenic trioxide all trans retinoic acid NB4, MR2 cell proliferation apoptosis differentiation
  • 相关文献

参考文献5

  • 1[1]Brown D,Kogan S,Lagasse E,et al.A PMLRARα transgene initiates murine acute promyelocytic leukemia[J]. Proc Natl Acad Sci USA,1997,94(6):2551-2556.
  • 2[2]Chen GQ,Zhu J,Shi XG,et al.In vitro studies on celluar and molecular mechanisms of arsenic trioxide(As2O3) in the treatment of acute promyelocytic leukemia : As2O3 induces NB4 cell apoptosis with downregulation of Bcl-2 expression and modulation of PML-RARα/PML proteins[J].Blood,1996,88(3):1052-1061.
  • 3[3]Chen GQ,Shi XG,Tang W,et,al.Use of arsenic trioxide(As2O3) in the treatment of acute promyelocytic leukemia(APLA): Ι.As2O3 exerts dose dependent dual effects on APL cells[J]. Blood,1997,89(9):3345-3353.
  • 4[4]Jing Y,Wang L,Xia L,et al.Combined effect of all-trans retinoic acid and arsenic trioxide in acute promyelocytic leukemia cells in vitro and in vivo[J].Blood,2001,97(1):264-269.
  • 5[5]Gianni M,Koken MH,Chelbi-Alix MK,et al.Combined arsenic and retinoic acid treatment enhances differentiation and apoptosis in arsenic-resistant NB4 cells[J]. Blood, 1998,91(11):4300-4310.

同被引文献15

  • 1Otsuka M, Kato N, Shao RX, et al. Vitamin K2 inhibits the growth and invasiveness of hepatocellular Carcinoma cell via protein kinase A activation[J].Hepatology,2004,40(1):243~251.
  • 2Miyazawa K,Yaguchi M,Funato K,et al. Apoptosis/differentiation-inducing effects of vitamin K2 on HL-60 cells: dichotomous nature of vitamin K2 in leukemia cells[J]. Leukemia, 2001, 15: 1111~1117.
  • 3Funato K, Miyazawa K, Yaguchi M, et al. Combination of 22-oxa-1,25-dihydroxyvitamin D3,a vitamin D3 derivative, with vitamin K2(VK2) synergistically enhances cell differentiation but suppresses VK2-inducing apoptosis in HL-60 cells[J].Leukemia,2002,16:1519~1527.
  • 4Yoshida T, Miyazawa K, Kasugo I, et al. Apotosis induction of vitamin K2 in lung carcinoma cell lines:the possibility of vitamin K2 therapy for lung cancer[J].Int J Oncol,2003,23(3):627~632.
  • 5Davis W, Lamson MS, Steven M, et al. The anticancer effect of vitamin K[J].Alternative Medinine Review,2003,8(3):303~318.
  • 6Chen G Q, Zhu J, Shi X G, et al. In vitro studies on cellular and molecular mechanisms of arsenic trioxide ( As2O3 ) in the treatment of acute promyelocytic leukemia : As2O3 induces NB4 cell apoptosis with downregulation of Bcl-2 expression and modulation of PML-RAR alpha/ PML proteins [ J]. Blood, 1996, 88 ( 3 ) :1052 -61.
  • 7Lallemand L V,Guillemin M C,Janin A, et al. Retinoic acid and arsenic synergize to eradicate in cells in a mouse model of acute promyelocytic leukemia [ J ]. Exp Med, 1999,189 ( 7 ) : 1043.
  • 8Jing Yongkui , Wang Long, Xia Lijuan,et al. Combined effect of all-trans retinoic acid and arsenic trioxide in acute promyelocytic leukemia cells in vitro and in vivo[ J]. Blood,2001, 97( 1 ) :264 -9.
  • 9Maurizio Giannì,Mareel H M Koken, Mounira K,et al. Combined arsenic and retinoic acid treatment enhances differentiation and apoptosis in arsenic-resistant NB4 Cells[J]. Blood , 1998 ,91 (11 ):4300 - 10.
  • 10Julie Mathieu, Stephane Giraudier, Michel Lanotte, et al. Retinoid-induced activation of NF-kB in APL cells is not essential for granulocytic differentiation, but prolongs the life span of mature cells[J]. Oncogene,2005,24:7145-55.

引证文献2

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部