摘要
介绍了一种快速、准确地测定3-(6-脱氧-6-磺酸基-α-D-吡喃型葡萄糖苷基)-sn-酰基甘油脂的方法。测定方法是基于苯酚和糖类能在浓硫酸快速加热下形成一种有色复合物。经过实验和统计学分析,结果表明酰基链对测定结果没有影响。该测定方法有较高的灵敏度和准确度,而且可以直接测定从薄层层析板获得的样品。
A rapid, sensitive spectrophotometeric method for quantitative determination of 3-(6-deoxy-6-sulfo-a-D-glu-copyranosyl)-sn-acylglycerol is described. The assay method is based upon the formation of a colored complex between phenol and saccharide heating with concentrated sulfuric acid. The experimental result and statistic analysis indicated that the acyl chain can not affect the result of determination.
The method is more sensitive than most methods currently used and has a high degree of precision. In addition, the method can be applied to samples obtained from thin-layer plates.
出处
《海洋科学》
CAS
CSCD
北大核心
2003年第6期55-58,共4页
Marine Sciences
基金
中国科学院知识创新工程领域前沿项目
