人工养殖点带石斑鱼弧菌病病原菌的分离及鉴定

CHARACTERISTICS OF PATHOGENIC BACTERIA OF VIBRIOSIS IN CAGED MALABAR GROUPER, Epinephelus malabaricus

广东、海南两省多个海水网箱养殖场养殖的点带石斑鱼 (Epinephelus malabaricus)发生严重的弧菌病,从海南三亚和广东深圳的发病鱼病灶上分离到 2株致病性细菌 VAL02和 VAL00,经人工回接感染实验证明这 2株菌引起点带石斑鱼弧菌病, VAL02对点带石斑鱼苗的半致死量是 2.25× 10 5 cfu/g.经形态及生理生化特征分析,鉴定出 VAL02及 VAL00均为溶藻弧菌.应用药敏纸片法研究了 22种化学疗剂对 VAL02及溶藻弧菌标准株的生长抑制作用,发现 VAL02对氯霉素、氟哌酸、卡那霉素和乙酰螺旋霉素等产生耐药性 ,对复方新诺明和环丙沙星等敏感.

Cage-cultured groupers have suffered from severe ulcerative disease in Guangdong and Hainan Province in the last two years. Two pathogenic vibrioes, strain VAL02 and VAL00 were isolated from outbreaks of vibriosis which caused death of malabar groupers, Epinephelus malabaricus in Sanya, Hainan Province in March 2001 and in Shenzhen, Guangdong Province in June 2000. They were identified as Vibrio alginolyticus on the basis of their characteristics and a number of biochemical tests. Strain VAL02's LD50 was 2.25 x 105 cfu/ g. It was sensitive to chemotheratpeutants such as sul-famethoxazole and cyprcoxacin, and was resistant to penicillin, norfloxacin and chloramphenicol.