利用基因枪法将玉米矮花叶病毒外壳蛋白基因导入玉米优良自交系

Transfer of MDMV CP Gene into Maize Elite Inbred Lines by Microprojectile Bombardment

利用基因枪法将玉米矮花叶病毒外壳蛋白基因导入玉米优良自交系18-599红、18-599白幼胚诱导的愈伤组织,转化的愈伤组织在Bialaphos浓度为8mg/L、10mgL、5mg/L的筛选压下经过三次抗性筛选后,分别再生出可育植株12株和6株。PCR检测结果表明18-599红和18-599白分别有10株和3株为阳性,说明CP基因已导入到玉米自交系中。

We introduced MDMV CP gene into calli derived from immature embryos of elite inbredlines18-599Hong and18-599Bai by microprojectile bombardment.12fertile transformants of18-599Hong and6fertile trans-formants of18-599Bai were obtained from resistant calli which had been subcultured on selecting medium contain-ing8,10and5mg /L Bialaphos for3cycles with3weeks per cycle.The result of PCR amplification implied that the CP gene had been introduced into maize inbred lines18-599Hong and18-599Bai.