灵光注射液对失血性休克大鼠胃肠粘膜的保护作用

Effect of Compound Anisodine on the Mucosa of Gastrointestinal Tract after Hemorrhagic Shock in Rats

目的 观察灵光注射液 (复方樟柳碱 )对失血性休克再灌注大鼠胃肠粘膜损伤的影响。方法 将 5 6只雄性Wistar大鼠随机分 4组 ,分别设为假休克组 (8只 )、模型组 (16只 )、灵光注射液低剂量组 (16只 )和高剂量组(16只 ) ,除假休克组外 ,大鼠均经历 4kPa ,70min的失血性休克 ,在休克复苏后 6h和 12h各组分别处死半数动物 ,观察大鼠肠道菌移位情况、病理组织学和超微结构变化。结果 灵光注射液对大鼠失血性休克再灌注引起的肠道细菌移位和胃肠粘膜形态损伤有明显的保护作用 ,其治疗机制可能与改善微循环。

Objective To observe the effect of LINGGUANG injection(compound anisodine)on the mucosa of gastrointestinal tract after hemorrhagic shock in rats Methods Fifty six male Wistar rats were divided into 4 groups randomly,which were sham shock,shock,shock treated with LINGGUANG injection in low dose and high dose groups separately. Animals were subjected to sham shock,or 70 minutes hemorrhagic shock at 4 kPa blood pressure with or without LINGGUANG injection treatment Half of the rats were sacrificed at 6 hour after resuscitation,the rest were killed at 12 hour Some organs were qualitatively cultured for translocating bacteria Stomachs,jejunums and ileums were examined under light microscope, and jejunum mucosas were observed under transmission and scan electron microscope Results and Conclusion Bacteria translocating to mesenteric lymph nodes,livers and spleens was less when treated with compound anisodine compared with control shock rats,the damages of gastrointestinal tract also became slighter in treated rats The mechanisms of compound anisodine treatment may be related with microcirculation improving and free radical scavenging