氯仿对非洲绿猴肾细胞损伤机理的研究

Study on the Injury Mechanism of Chloroform to Vero Cells

目的 实验研究氯仿染毒 2 4h对非洲绿猴肾细胞的损伤及其机理。方法 运用显微荧光术测定了非洲绿猴肾细胞 (Vero细胞 )内活性氧 (ROS)含量及游离Ca2 + 浓度 ,同时 ,测定Vero细胞培养上清液乳酸脱氢酶(LDH)活力用于检查Vero细胞受损情况。结果 接触浓度为 4 0mmol L氯仿的Vero细胞内ROS含量及游离Ca2 +浓度与对照组比较无显著性差异 (P >0 0 5 ) ,同时 ,表示Vero细胞受损指标 (LDH活力 )也无显著性差异 (P >0 0 5 ) ;而接触浓度为 8 0mmol L、12 0mmol L氯仿的Vero细胞内ROS含量显著高于对照组 (P <0 0 1) ,其Vero细胞受损也显著增加 (P <0 0 5、P <0 0 1)。结论 较高浓度的氯仿能损伤Vero细胞 。

Objective To understand the injury mechanism of Vero cells(African green monkey kidney cells) exposing to chloroform(CCl 3)for 24hours.Methods\ The content of reactive oxygen species(ROS) and cytoplasmic Ca 2+ concentration in Vero cells were measured with microfluorometry. Meanwhile, the injury of Vero cells was detected with lactate dehydrogenase(LDH) activity in the supernatant of the medium for Vero cells. Results\ There were no any differences for the above indices between CCl 3 group of 4 0mM concentration and control group statistically( P >0 05). Moreover, there were no any differences in cytoplasmic Ca 2+ concentration of Vero cells between all groups exposing to CCl3 and control group( P >0.05). In the other hand, the contents of ROS in the Vero cells exposing to CCl 3 at 8 0mM and 12 0mM concentration were higher than those in the control group( P <0 01)respectively, and the activity of LDH in supernatant of the medium for Vero cells exposing to CCl 3 at 8 0mM and 12 0mM concentration was higher than that of latter( P <0.05, P <0 01)respectively. Conclusion\ The possible injury mechanisms of Vero cells exposing to CCl 3 of high concentration are partly mediated by increasing the content of ROS in Vero cells.