荞麦花粉黄酮对氧自由基致鼠红细胞膜氧化损伤的保护作用

The Protective Action of Buckwheat Pollen Flavones on Oxidative Injury of Erythrocyte Membrance Induced by Oxygen Radicals

应用1,6-二苯基-1,3,5-已三烯(DPH)为荧光探针,研究了荞麦花粉黄酮对超氧阴离子自由基和羟基自由基导致鼠红细胞膜氧化损伤的影响。结果表明,超氧阴离子自由基和羟基自由基均能引起鼠红细胞膜脂质过氧化反应,脂质过氧化产物丙二醛含量显著升高,膜脂流动性下降。将鼠红细胞膜预先用荞麦花粉黄酮处理后,膜脂的MDA含量明显下降,呈现剂量与效应关系,膜脂流动性显著提高,表明荞麦花粉黄酮是一种天然抗氧化剂,能防止超氧阴离子自由基和羟基自由基引起的鼠红细胞膜损伤。

1,6-diphenyl-1,3,5-hexatnene, a kind of fluorescence probe, was used to observe the effects of buckwheat pollen flavones on the oxidative damage in rat erythrocyte induced by peroxy radical and hydroxy radical.The results demonstrated that both peroxy radical and hydroxy radical increased the content of malondialdehyde( MDA), decreased membrance fluidity significantly, and caused lipid peroxidation of rat erythrocyte .But buckwheat pollen flavones decreased the content of MDA remarkably, improved the membrance fluidity of rat erythrocyte and inhibited the lipid peroxidation in rat erythrocyte membrance. These results suggested that buckwheat pollen flavones was a kind of natural antioxidant and could prevent lipid peroxidation in rat erythrocyte membrance damage induced by peroxy radical and hydroxy radical.