姜黄素在肾毒血清肾炎中防治作用的初步研究

The Effect of Curcumin on Nephrotoxic Serum Nephritis in Rats

目的:探讨姜黄素对大鼠肾毒血清肾炎的防治作用,为将其应用于临床治疗肾小球肾炎提供实验依据。方法:将雄性SD大鼠随机分成3组:①正常对照组:从尾静脉注射0.5ml/d生理盐水,连用2日;②模型组:从尾静脉注射免抗鼠肾毒血清0.5ml/d,连用2日;③姜黄素组:于尾静脉注射免抗鼠肾毒血清0.5ml/d,连用2日,同时腹腔注射姜黄素50mg·kg^(-1)·d^(-1)。3组大鼠分别于第3、7、14、28天处死,测定各批次大鼠24h尿蛋白,并观察肾脏病理组织学改变。结果:姜黄素组24h尿蛋白明显低于模型组(P<0.01),半定量分析显示,姜黄素组肾小球细胞数明显低于模型组(P<0.01);间质炎细胞浸润亦少于模型组(P<0.01);14天后肾小管间质损伤指数姜黄素组也明显好于模型组(P<0.01)。结论:姜黄素能明显减轻肾毒血清肾炎蛋白尿程度,抑制肾小球内细胞增殖,减轻肾小管间质损伤及炎细胞浸润,具有肾脏保护作用。

Objective: To investigate the effect of curcumin on nephrotoxic nephritis in rats. Methods: Healthy male Sprague-Dawley rats were randomly divided into three groups, 24 animals for each group. The rats of normal control group were injected introvenously with normal saline 0. 5 ml/d for two days. The rats of nephrotoxic serum nephritis (NTN) group were injected intravenously with 0. 5 ml/d nephrotoxic serum for two days. The rats of curcumin treated group were injected introvenously with 0. 5 ml/d nephrotic serum for two days, and meanwhile received 50 mg · kg-1·d-1 of curcumin intraperitoneally every day. Animals were killed on days 3, 7, 14, 28 in batch and the samples of urien and kidney were collected. Twenty-four hours urine protein was measured by sulf-salicylic acid method and medical imaging analysis system was performed to assess the pathological damage of the kidney. Results: Treatment of NTN rats with curcumin resulted in less proteinuria ( P < 0. 01), less interstitial tubular damage ( P < 0. 01) and attenuation of the cell proliferation in glomerulus as compared with those in NTN animals without curcumin treatment ( P < 0. 01). Conclusion: Curcumin reduces renal injury induced by anti-nephrotoxic serum.