胃癌组织微卫星不稳定性的研究

Microsatellite Instability in Gastric Cancer

目的:探讨微卫星不稳定性(MSI)在胃癌中的发生规律及其与胃癌临床病理的关系。方法:对30例胃癌及癌旁正常组织石蜡切片提取DNA,应用银染PCR-SSCP技术检测5个MSI。结果:胃癌组织MSI的发生率为23.3％,其中高频MSI(MSI-H)3例,低频MSI(MSI-L)4例。5个位点中以BAT-26的阳性率最高,为13.3％。胃窦癌MSI的发生率显著高于贲门癌(P<0.05),MSI与性别、年龄、细胞分化程度、淋巴结转移、肿瘤TNM分期无关。结论:MSI的发生率与所选位点有关;MSI更多见于胃窦癌,MSI在胃癌的发生发展中可能具有重要作用。

Objective:To observe the changeable patterns of microsatellite instability (MSI) in sporadic gastric cancer and the relationship between MSI and clinicopathological features. Methods: Silver staining single strand conformation polymorphism-polymerize chain reaction (PCR-SSCP) was used to screen MSI markers at 5 loci in formalin-fixed, paraffin-embeded tissues of sporadic gastric carcinoma ( n = 30) and corresponding non-cancerous gastric tissues. Results:The abnormal shifting of the single-strand DMA was identified in 7 out of 30 cases (23. 3% ) of sporadic gastric cancer. Frequency of MSI at BAT-26 was higher than that at the other loci. Gastric cancer with MSI was associated with location of the tumors, but no association was observed between MSI status and age, sex, differentiation, lymph node metastasis and TNM stage. Conclusion: Gastric cancer with MSI is more likely to be located in the antrum of stomach. MSI may play an important role in the carcinogenesis of sporadic gastric cancer.