儿童支气管哮喘96例血清IL-13的研究

Determination of Serum IL-13 in Children with Bronchial Asthma

目的:探讨血清IL-13水平与儿童哮喘的关系。方法:用ELISA法检测血清IL-13、总IgE水平,采用荧光酶联免疫法测定嗜酸性粒细胞阳离子蛋白(ECP)水平,同时计数外周血嗜酸性粒细胞(EOS),并对检测结果进行统计学处理。结果:哮喘组患儿血IL-13水平与正常对照组比较差异有显著性(P<0.001),哮喘组血IL-13水平与ECP、总IgE及EOS计数均呈正相关。结论:血IL-13水平与儿童哮喘发病间可能存在着密切的关系。

Objective: To explore the relationship between serum IL-13 and other blood indices in children with asthma. Methods: The serum IL-13 and total-IgE were detected by the ELISA method, the levels of serum eosinophil cation protein (ECP) were determined by fluoresent enzyme-linked immunoassay, the numbers of blood eosinophil (EOS) were counted in asthmatic or normal children. The results of determination were analysed statistically. Results: The serum IL-13 from asthmatic children was higher than that of the normal control group (P < 0. 001) and the serum IL-13 levels were positively correlated to the levels of ECP, total-IgE and the number of EOS. Conclusion: IL-13 may be in close relationship with pathogenesis of asthma in childhood.