摘要
利用自行组装的激光诱导荧光 CCD阵列检测器 计算机联用装置 ,建立了测定食品中锌的灵敏方法。在乙酸 乙酸铵缓冲溶液 (pH 4 )中 ,Zn(Ⅱ )与SCN- 、若丹明B(RhB)反应生成Zn SCN RhB配合物 ,经乙醚萃取后 ,在激发波长 5 32nm和发射波长 5 80nm进行荧光强度测定。研究了表面活性剂对Zn SCN RhB配合物荧光强度的影响 ,优化了荧光反应及食品样品的处理条件 ,并用于食品试样中锌的测定。方法的检出限为 4ng·ml- 1,加标回收率为 90 .0 %～ 110 .0 % ,相对标准偏差为 2 .0 %～ 9.7% ,能满足食品卫生检验的要求。
A sensitive method for determination of zinc in food by laser induced fluorophotometry with CCD array detectors was developed. In a sodium acetate buffer medium (pH 4), Zn(Ⅱ) reacts with SCN - and rhodamine B (RhB) to form a complex of Zn SCN RhB, which is extracted into ether and its fluorescence intensity is measured at λ ex 532nm and λ em 580nm. The conditions of the fluorescence reaction, including the pretreatment of food samples, the effect of surfactant on the fluorescence intensity of the ternary complex and etc were optimized. The limit of detection of this method is 4ng·ml -1 . Recoveries tested by standand addition method were in the range of 90 0%～110.0% and the relative standard deviations varied from 2.0% to 9.7%. The results obtained by the proposed method were in good agreement with those of AAS method. This method has been successfully applied to food analysis.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2003年第6期336-338,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家自然科学基金 (No .3 9770 64 6)
国家教委博士点基金 (No.9760 )资助课题
