独角莲对肝癌细胞SMMC-7721细胞增殖抑制作用机理的研究

THE INHIBITION EFFECTS OF TYPHONIUM GIGANTEUM ENGl. ON HEPATOCARCINOMA CELL

临床上独角莲对包括肝癌在内的多种肿瘤有一定的治疗作用。为揭示其抑癌机理,我们用独角莲根茎水提物(the aqueous extract from dried powdered rhizomes of Typhonium gigante-um Engl.,AEoTGE)作用肝癌细胞株SMMC-7721,研究独角莲对SMMC-7721细胞增殖、细胞周期和细胞凋亡的影响。噻唑氮蓝(MTT)比色实验和流式细胞仪(flow cytometry,FCM)测定表明:AEoTGE能较强抑制SMMC-7721细胞的生长,SMMC-7721细胞被阻滞在S期,并诱导细胞凋亡。提示独角莲是一种在肝癌治疗上有前景的中草药。

Typhonium giganteum Engl. has been used to treat many cancers for many years in China. This research was designed to study the inhibition of the a queous extract from dried powdered rhizomes of Typhonium giganteum Engl. (AEoTGE)on the proliferation, cell cycle and apopto-sis of hepatocarcinoma cell SMMC-7721. MTT color-metric assay and flow cytometry results showed: AEoTGE can decrease the proliferation rate of SMMC-7721 cells; the significant S phase arrest was revealed by the increase of cells in S phase and the obvious apoptosis of SMMC-7721 cells was induced by AEoTGE. Taken together, these results suggest that Typhonium giganteum Engl. is a promising herbal drug in the therapy of hepatocellular carcinoma.