促红细胞生成素对大鼠局灶性脑缺血再灌注后梗死体积和脑组织水肿的影响

The effects of erythropoietin on histological changes of cerebral infarction size and brain tissue water content after focal cerebral ischemia-reperfusion in rats

目的 探讨促红细胞生成素(EPO)对大鼠脑缺血再灌注损伤的保护作用。方法 采用阻断大鼠一侧大脑中动脉(MCA)血流2小时,再灌注48小时制成局灶性脑缺血模型。于再灌注开始前,治疗组经腹腔注入EPO(3000 U/Kg);缺血组和假手术组给予生理盐水腹腔注射,48小时后断头取脑。制作 HE切片;以2％氯化-2,3,5三苯基四氮唑(TFC)对脑片进行染色;经图像分析仪计算梗死体积占全脑体积的百分比;同时用干湿重法测定脑组织的含水量。结果 与对照组相比,治疗组海马 CA_1区神经细胞减少(25.7±1.16)％,缺血组减少(31.2±1.49)％;治疗组与缺血组比较有显著性差异(P<0.01)。治疗组脑梗死体积比缺血组明显缩小(P<0.01)。治疗组脑组织含水量比缺血组明显减少(P<0.01)。结论 EPO能够显著降低脑组织含水量,抑制脑水肿,缩小脑梗死体积及减少神经细胞坏死,对脑缺血再灌注损伤有良好的保护作用。

Objective To explore the protective effects of erythropoietin (EPO) on the histological changes of cerebral infarction volume and brain tissue water content after focal cerebral ischemia-reperfusion in rats. Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery(MCA) for 2h and reperfusing for 48h. The rats received either EPO (3,000units/kg of bodyweight) or saline i.p. just before the beginning of reperfusion and the brains were removed after 48h.Some brain tissues were used for the observation of cell death with the method of HE staining. Some brains were made into sections stained by 2 % triphenyltetrazolium chloride (TTC), the percent of the cerebral infarction volume to whole cerebral volume was calculated by using image analysis system. The cerebral water content was measured by the method of dry-wet weigh. Results At 48h after ischemia-reperfusion, compared with control group, the neurons in the hippocampal CA1 region in treatment group and ischemia group were reduced by 25 .7 ±1 .16% and 31 .2±1.49% respectively, There was a remarkable difference between treatment group and ischemia group (P < 0.01). Compared with ischemia group, the cerebral infarction volume in treatment group was significantly smaller( P < 0.01) ,and the cerebral water content was also decreased significantly. Conclusion EPO could reduce the neurons necrosis and the cerebral infarction volume and decrease the cerebral water content. So EPO has a protective effect on ischemia-reperfusion injury.