金属离子Cu^(2+),Co^(2+),Ni^(2+)的微芯片电泳分离及化学发光检测

Microchip Electrophoretic Separation of Cu^(2+), Co^(2+)and Ni^(2+) with Chemiluminescent Detection

根据流动注射 -化学发光的通用实验装置图 ,设计并通过标准光刻技术 ,湿化学刻蚀及热键合技术制作了玻璃微芯片 .在该芯片上将毛细管电泳分离与化学发光检测相联用 ,鲁米诺和H2 O2 化学发光反应溶液通过实验室自制的微流泵输送 .对所用电压 ,缓冲溶液和发光试剂流速等实验条件进行了优化 .在所选的最佳条件下 ,成功地实现了金属离子Cu2 + ,Co2 + ,Ni2 + 的电泳分离 -化学发光检测 ,检测限分别为 5 0× 10 -9,5 0× 10 -11和 1 0× 10 -7mol/L .

A glass microchip was designed on the base of flow injection-chemiluminescent devices and fabricated by standard photolithgraphy technology, wet chemical etching and heat bonding technology. Electrophoresis separation was cooperated with chemiluminescent detection on the microchip, and the chemiluminescent reagents of luminol and hydrogen peroxide solution were delivered by a laboratory-made microfluidic pump. The experimental conditions including buffer applied, voltage and the flux of the chemiluminescent reagents were optimized. Under the optimal conditions, Cu2+, Co2+ and Ni2+ were separated and detected with chemiluminescent method on the chip. The detection limits of Cu2+, Co2+ and Ni2+ can reach 5.0 x 10(-9), 5.0 x 10(-11) and 1.0 x 10(-7) mol/L, respectively.