冠突伪尾柱虫休眠与营养细胞线粒体DNA的比较

Polymorphic Comparison between the Mitochondrial DNA of Vegetative Cell and Resting Cyst in Pseudourostyla Cristata

为研究纤毛虫休眠状态下线粒体的行为特征及功能与纤毛虫形成包囊的关系,采用RAPD技术对冠突伪尾柱虫休眠细胞与营养细胞的线粒体DNA进行了比较。结果表明,在选用的15条随机引物中,有5条引物的扩增产物完全相同,其余10条引物的扩增产物各有差异。15条引物共扩增出84条片段,其中共享片段为28条,共享度为66.67％。这说明休眠细胞线粒体DNA与营养细胞线粒体DNA在很大程度上是一致的,但仍存在一定差异,据此推测细胞在形成包囊的过程中,某些线粒体DNA结构可能发生了改变,这些改变可能与线粒体在包囊内的行为特征及功能有关。

In order to study the relationship between vegetative cell and resting cyst, RAPD method was selected. The result revealed that; the polymorphic fragments amplified with primers S176,S267,S217, S259 and S319 share the same fragments, but the other primers such as S283,S180,S61,S64,S17,S55, S38,S30,S23 and S28 have deferent amplied fragments. There are 84 fragments were amplified by 15 random primers. In these fragments, both vegetative cell and resting cyst mtDNA have 28 similar fragments. The similarity values is 66.67%. It is implied that though there is similarity between mtDNA of vegetative cell and resting cyst, some changes may occurred in resting cyst mtDNA, these changes may be related to the function of resting cyst mitochondrion.