摘要
建立了血中胺碘酮的胶束电动毛细管电泳分析方法 (MEKCE)。采用未涂层融硅毛细管分离通道 ,以 2 5mmol/L十二烷基硫酸钠 (SDS) 5 0mmol/L硼酸 三 (羟甲基 )胺基甲烷 (Tris)缓冲液 (pH 8.33)为电泳介质 ,6.9KPa压力进样 ,2 5kV恒压电泳 ,2 5℃电泳温度 ,2 4 3nm检测波长。线性范围 7.2～ 72 0 0ng/mL(r=0 .9934) ,血药浓度的最低检出量 72 .0ng/mL ,血清标本的平均回收率为5 0 6% ,RSD为 1 .2 %。
The determination of amiodarone in blood by micellar electrokinetic capillary electrophoresis(MEKCE) with sodium dodecyl sulphate(SDS) as the micellar phase was described. Separation column: bare fused sillica capillary, electrolyte: 25 mmol/LSDS 50 mmol/L borax tris (pH 8.33),detection wavelength: 243 nm,sampling pressure: 6.9 KPa,running voltage: 25 kV, temperature:25 ℃. The linear range of determination for amiodarone was 7.2～7200 ng/mL ( r =0 9934) with a detection limit of 72 ng/mL. The RSD of recovery testing was 1.2%. The method was used for clinic determination of amiodarone in human serum.
出处
《分析试验室》
CAS
CSCD
北大核心
2003年第2期64-66,共3页
Chinese Journal of Analysis Laboratory
基金
浙江省教育厅科研计划项目 (2 0 0 0 0 2 6 7)
